BioLight Toxy

The new and innovative Aqua Science BioLight Toxy brings rapid toxicity testing to the twenty-first century. The Toxy is a versatile system, designed to be used interchangeably as either a field portable or desktop instrument.

Advanced Technology, Versatility, and Performance for Toxicity Testing

Versatile: Modular bench-top and portable reader in one
Expandable: Bench top offers up to 2 block per unit
Multilingual: More than 5 languages included and more coming
Customizable: Fully Customizable Protocols from Start to Finish
Reliable: State of the art materials and components – Aluminum & Steel
Compatible: Meets All ISO 11348-3 and NMX-AA-112-SCFI-2017 requirements
Effective: Streamlined workflow for efficient and accurate toxicity testing.
Independent: No need of tablet or computer to run.
Accessible: Remote access
Rapid: Read time is <7 seconds
Value: Most cost-effective offering in the industry
Convenient: Local Maintenance and Technical Support

Biolight-Toxy-Versatile

How it works

The BioLight Toxy measures the light levels of AliiVibrio fischeri to determine the toxicity of a sample. Results are reported in percent effect or EC50 (Effective Concentration 50%). This measurement can then be easily converted to UT (Unity of Toxicity)—the unit required by the New Norm 001.

Using the BioLight Toxy along with Aqua Science BioLight Multi or Single Reagents and consumables provides testing that can screen over 3,600 chemical compounds simultaneously, while also detecting synergistic toxicity.

Aqua Science BioLight Toxy delivers rapid toxicity testing with specialized reagents and consumables

It offers the most up to date, dual option instrument available for bioluminescent analysis in the market. This is a result of matching a team of experts in ecotoxicity to fulfill the needs of toxicity users around the world.

This state-of-the art luminometer brings together the capabilities of doing portable testing in the field as well as bench top testing in the lab. This all-in-one unit is designed to be used in conjunction with the BioLight Multi and Single Reagent as well as BioLight salinity adjusting solution, BioLight diluent and BioLight Recon. The BioLight Toxy instrument is used to read the differences in light from time zero and after exposure to a sample which could be toxic.

Biolight Toxy
Biolight-Portable

BioLight Toxy Portable

When using the BioLight Toxy in the field, two standard protocols are set up in the onboard software, the Primary Test and the Quick Test. This testing requires the BioLight Single Test Reagent, BioLight Diluent, BioLight Salinity Adjusting Solution and disposable glass cuvettes.

The BioLight Toxy field portable mode does not include temperature control, so it is important to keep the reagent frozen until use. Each vial of Single reagent will do up to 3 tests. Typically, this will be a negative and positive control and 1 sample. To start, sample tubes are set up in a cuvette rack based on the number to be analyzed. Following the protocol in the instrument, the reagent will be prepared and will require a 15-minute wait time to equilibrate. Step by step instructions will be provided in the software protocol for sample prep and reading. The BioLight Toxy will read the Single reagent at time zero to have a baseline to compare the readings after sample exposure. The instrument can be set for custom read times, however the typical field read time is 5 minutes.

This test will provide results in 20 minutes total. The Toxy can be set to read again at 15 minutes if required to see if there is a significant difference in the light loss. Different chemicals often have effects on the light change at different times. Light loss may be present at 5 minutes but may continue to decrease at 15 minutes. The 15-minute reading may be more reliable.

Biolight Toxy Bench Top

The BioLight Toxy bench top instrument includes a cooled read well and cooled reagent well along with a separate cooling block to ensure all samples are kept at the appropriate temperatures during testing. There is also the option to add an additional cooling block to double the number of samples that can be tested.

Additionally required to perform the analysis are the BioLight Multi Reagent, BioLight Diluent, BioLight Recon, BioLight Salinity Adjusting and disposable glass cuvettes.

When using the BioLight Toxy bench top, there will be multiple protocols available to choose in the onboard software. The software is easy to use and provides all results calculations that can be printed or uploaded to a computer as well as saved in the software. Choosing a protocol will be based on the water type, expected toxicity level and results of the baseline data if available. Most protocols provide results in approximately 45 minutes, including the 15 minutes for the BioLight Multi reagent to equilibrate after reconstitution.

Software Biolight

Tech

  • 16 GB Storage
  • USB/Wifi (Optional)
  • SCADA Connectivity
  • Read time <7 seconds

Software

The Toxy features a custom PLC software interface on-board

User can either choose from a library of common toxicity tests already pre-programmed in the software or create a fully customized test that can be saved to the library for future testing. This is just one of many helpful features that have been incorporated into this new state-of-the art luminometer, making rapid acute toxicity testing even easier.

Software is multilanguage and friendly for new users.

Protocols

EC50 Test – 2%, 45% or 81.9%

Testing that provides a calculated EC50 value and measure relative toxicity.

  • 2% EC50 Test – samples expected to have a high level of toxicity. Typically, this would be for wastewater influent or pure compounds.
  • 45% EC50 Test – samples of unknown toxicity. This EC50 test measures relative toxicity and is the most adaptable. It is a good option for septage, influent wastewater and water from a treatment digester.
  • 81.9% EC50 – samples expected to have low to medium toxicity. Primarily used for drinking water, wastewater effluent, pore water and stormwater. The expected EC50 level for this test should be between 30% to 50%.

% Effect Test – 2%, 45% or 81.9%

Testing using a single concentration. This test will not determine relative toxicity or provide a dose response curve.

  • 2% Effect Test – Used for samples with high toxicity. Wastewater influent, septage, industrial trucked waste.
  • 45% Effect Test – Used for samples of medium toxicity.
  • 81.9% Effect Test – Used with samples of low toxicity such as drinking water.

Comparison Test

Used to compare the relative acute toxicity of an undetermined sample with a control sample. A control sample could be a sample of a determined toxicity. This is the best test for the analysis of low toxicity samples if the EC50 values can’t be achieved using the EC50 test.

ISO

Complies with ISO 11348-3. The ISO Standard states that the method is applicable for wastewater, fresh water – both surface and ground, sea and also brackish water, elutes of sediments, pore water – both aqueous extracts and leachates.

Solids Test

Used for soils, sediments and other solid samples. The test uses solid samples which are serial diluted with 9.9% being the highest concentration. It can be used for freshwater, marine and estuarine samples with the appropriate diluent. It allows for the detection of toxicity due to the insoluble solids that are not in solution by allowing the test organisms to come in direct contact with the solid sample in an aqueous suspension.

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